To be completed by the end of November 2009
| 6 | Lytham St. Annes | Home win | Culcheth |
| 7 | Penrith | 2 | v | 1 | Bury |
| 8 | Oswestry | 4 | v | 3 | Wilmslow |
| 9 | Chester | 2 | v | 8 | Tamworth |
| 10 | Selby District | 2 | v | 1 | Withernsea & District |
| 11 | Dumfries | 5 | v | 2 | Teesdale |
| 12 | Scarborough | 4 | v | 0 | Thirsk & North Allerton |
| 29 | Berkhamstead | 1 | v | 7 | Fairlop |
| 30 | Wokingham | 4 | v | 0 | Wimborne & Ferndown |
| 31 | Shirley | 10 | v | 0 | Rugby Rokeby |
| 32 | Barwell & Earl Shilton | Home win | Birmingham Sheldon |
| 33 | Guildford | 13 | v | 2 | Littlehampton District |
| 34 | Brighton | Home win | Horley |
| 35 | Whitstable & Herne Bay (Herne Bay) | Home win | Crowborough & District |
| 36 | Darent Valley | 9 | v | 0 | Whitstable & Herne Bay (Tankerton) |
| 37 | Ilfracombe | 4 | v | 2 | Glastonbury & Street |
| 38 | Newquay | Home win | Plymouth |
| 39 | South Molton (NMSC FC) | 7 | v | 2 | Seaton & District |
| 40 | Torpoint & Rame Peninsular | 5 | v | 0 | South Molton (NMSC Stags) |
| 41 | Bye | | v | | Frome |
| 42 | Llanwit Major | 2 | v | 0 | Leominister |