To be completed by the end of February 2010
| 13 | Penrith | | v | | Lytham St. Annes |
| 14 | Oswestry | 0 | v | 2 | Tamworth |
| 15 | Selby District | 5 | v | 0 | Tickhill & District |
| 16 | Scarborough | 4 | v | 1 | Dumfries |
| 17 | Notts Derby Hemlockstone | 2 | v | 9 | Bradgate |
| 18 | The Deepings | 1 | v | 6 | Loughborough |
| 19 | Louth | | v | | BYE |
| 20 | Glandford & Lindsey | Away win | Retford |
| 43 | Fairlop | 10 | v | 1 | Wokingham |
| 44 | Shirley | | v | | Barnwell & Earl Shilton |
| 45 | Brighton | 1 | v | 10 | Guildford |
| 46 | Whitstable & Herne Bay (Herne Bay) | Away win | Darent Valley |
| 47 | Torpoint & Rame Peninsular | 3 | v | 0 | Ilfracombe |
| 48 | Newquay | | v | | South Molton (NMSC FC) |
| 49 | Frome | 1 | v | 4 | Llantwit Major |
